![]() ![]() ![]() OBITools seems to have very nice documentation and is used widely, which is quite useful in case I have questions or get stuck. Also, by writing and owning your own scripts you get a really good sense of what you’re doing, and you can easily replicate your work (in other words, you can just re-run your script instead of changing all the settings in an interface). However, I know that lot of bioinformatics folk are very happy using the UNIX command line interface for their work. ![]() The Primer3 website, or the implementation by NCBI called Primer-BLAST, seems very accessible if you’re used to using software with a graphical interface. The two options that seem to be widely used are the Primer3 website or the OBITools tool called ecoPrimers (see ‘ software for eDNA‘ for more information). So, the very first step in my metabarcoding research (after literature research of course!) was to design a species-specific primer. ![]()
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